Differential Staining BIOS242 Week 3 Lab 5

BIOS242, Week 3, Lab 1 Name: Lab 5: Differential Staining Learning Objectives:  Learn the principle of differential staining  Learn how to carry out a procedure of differential staining to identify bacteria Differential stain uses two or more stains to specifically stain certain structures or cellular components which cannot be easily observed using simple stains. Differential staining principles are based upon the specific chemical nature and composition of cellular components and therefore, different structures are observed using different stains and staining Bacteria with Bacteria with procedures. Differential staining often thin cell wall thick cell wall becomes the basis of identification of the Primary Stain: Crystal bacteria in clinical labs. Violet Mordant: Iodine In this lab, we will learn about four separate staining procedures. Decolorizing agent: Acetone/Alcohol Gram negative cell Counterstain: Safranin Gram positive cell     Gram Staining Endospore Staining Acid-fast Staining Capsule Staining (Special Staining) Exercise 1: Gram Staining Gram staining is used to identify cells based on the differences of the cell wall. While some bacteria may contain a thick layer of peptidoglycan that forms a cell wall, othe