Lab Exam BIOD -171

1. What three elements are used in an autoclave to sterilize equipment? 2. What is the minimum temperature an autoclave must be set at to achieve sterile condition? 3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer. 4. What type of incubator is pictured below? Answer the following questions. 1. At what temperature is the fixed incubator set to, as presented in the lab video? 2. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial. 3. What are the FOUR types of gloves presented in the lab video? 4. What THREE rules were discussed in regards to lab safety that would protect you and others from contamination? Answer the following questions. 1. You are a lab instructor and Paul Smith has turned in his lab notebook for you to grade the lab experiment #2 on microscopy. Based upon what was covered in the lab video, how should Paul have entitled his lab experiment? 2. You arrive to your first day of work at a new lab. You are taking over for someone who took a new job at another lab. Your boss informs you that because of time restraints, this person did not exactly follow the experimental protocol. In order to proceed, you must know what he did differently. (1) According to the lab lecture, under what section of his notebook would you look to find the experimental steps? (2) As changes to the experimental steps were made, what are these differences called and how should it appear in the lab notebook? Answer the following questions 1. Identify the part of the microscope indicated by the arrow. 2. Identify the part of the microscope indicated by the arrow. 3. Identify the part of the microscope indicated by the arrow. Answer the following questions 1. You are about to study a bacterial sample under a light microscope. You look into the oculars and see two circles. What adjustments need to be made? 2. What 2 parts of the microscope contributes to the total magnification to your sample? 3. As you looking through the microscope you wish to dim (or limit) the amount of light entering into the eyepiece—what component of the microscope other than the light source itself can be adjusted to make these modifications?