Microbiology LAB EXAM #1

LAB EXAM #1 1. What three elements are used in an autoclave to sterilize equipment? heat, pressure, and steam 2. What is the minimum temperature an autoclave must be set at to achieve sterile condition? 125°C 3. If you are working in a lab in which an autoclave is not available, and you are pressed for time, which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you chose your answer. Hot steam is the best choice as you can achieve a sterile environment in a matter of minutes whereas hot air will take several hours to achieve the same effect. 4. What type of incubator is pictured below? Fixed incubator 5. At what temperature is the fixed incubator set to, as presented in the lab video? 37°C 6. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial. 4°C. This temperature slows bacterial growth and prolongs the life of the sample. 7. What are the FOUR types of gloves presented in the lab video? Latex, Nitrile, Thermal cold, Thermal heat 8. What THREE rules were discussed in regards to lab safety that would protect you and others from contamination? 1. Never eat or drink in the lab 2. Always wear appropriate PPE 3. Never leave the lab wearing PPE 9. What are the main sections that should be found in a lab notebook? Name at least 4. Objective, Procedure, Notes, Results and Deviations LAB 2 4. How should you hold the microscope when you move it or carry it? You should hold it with one hand on the neck and one hand under the base 5. List the four magnification strengths found on the objectives of the light microscope shown in the lab video. 4x, 10x, 40x, 100x